The fUSI system enables 3 types of measurements:
- absolute and relative quantification of cerebral blood in both superficial and deep brain vascular networks
- structural imaging of the brain
- imaging of brain activity via cerebral blood volume changes throughout the entire rodent brain during behaviour
fUSI enables functional MRI-like imaging, without the MRI and has been demonstrated in mice during freely-moving behaviours. It represents a critical tool to evaluate vascular networks brain-wide in response to stroke, vascular dementia, Alzheimer disease, etc., particularly in subcortical areas implicated in various disease states. Structural imaging can be used to assess changes in response to edema.
|Imaging Depth||up to whole mouse brain (>2cm)|
|Data Analysis||large data; requires arivis Vision4D software|
Urban, A. et al. Real-time imaging of brain activity in freely moving rats using functional ultrasound. Nat. Methods 12, 873–878 (2015).
Macé, É. et al. Functional ultrasound imaging of the brain. Nat. Methods 8, 662–664 (2011).
Macé, É. et al. Whole-Brain Functional Ultrasound Imaging Reveals Brain Modules for Visuomotor Integration In Brief Article Whole-Brain Functional Ultrasound Imaging Reveals Brain Modules for Visuomotor Integration. Neuron 100, (2018).
Rungta, R. L., Osmanski, B. F., Boido, D., Tanter, M. & Charpak, S. Light controls cerebral blood flow in naive animals. Nat. Commun. 8, (2017).
Demené, C. et al. Transcranial ultrafast ultrasound localization microscopy of brain vasculature in patients. Nat. Biomed. Eng. 5, 219–228 (2021).